{"id":4096,"date":"2019-07-31T16:50:14","date_gmt":"2019-07-31T08:50:14","guid":{"rendered":"\/\/www.562part.com\/product\/hydroxyphenyl-fluorescein-hpf-%e7%be%9f%e8%8b%af%e5%9f%ba%e8%8d%a7%e5%85%89%e7%b4%a0\/"},"modified":"2023-09-15T09:55:15","modified_gmt":"2023-09-15T01:55:15","slug":"hydroxyphenyl-fluorescein-hpf-%e7%be%9f%e8%8b%af%e5%9f%ba%e8%8d%a7%e5%85%89%e7%b4%a0","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/hydroxyphenyl-fluorescein-hpf-%e7%be%9f%e8%8b%af%e5%9f%ba%e8%8d%a7%e5%85%89%e7%b4%a0\/","title":{"rendered":"Hydroxyphenyl fluorescein (HPF) \u7f9f\u82ef\u57fa\u8367\u5149\u7d20"},"content":{"rendered":"

Hydroxyphenyl fluorescein (HPF) \u7f9f\u82ef\u57fa\u8367\u5149\u7d20<\/strong><\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u4fe1\u606f<\/strong><\/span><\/p>\n

\n\n\n\n\n
\u4ea7\u54c1\u540d\u79f0\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/td>\n\u4ea7\u54c1\u7f16\u53f7<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
Hydroxyphenyl fluorescein (HPF) \u7f9f\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/td>\nMX4805-1MG<\/span><\/td>\n1mg<\/span><\/td>\n2180<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

 <\/p>\n

\u4ea7\u54c1\u63cf\u8ff0<\/strong><\/span><\/p>\n

\u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20\uff08APF\uff09\u548c\u7f9f\u82ef\u57fa\u8367\u5149\u7d20\uff08HPF\uff09\u662f\u7531T. Nagano\u5f00\u53d1\u7684\u4e24\u79cd\u6d3b\u6027\u6c27\uff08Reactive oxygen species, ROS\uff09\u6307\u793a\u5242\uff0c\u6bd4H2DCFDA\uff08DCFH-DA, DCFH\uff09\u5177\u66f4\u4f18\u79c0\u7684\u7279\u5f02\u6027\u548c\u7a33\u5b9a\u6027\u3002APF\u548cHPF\u663e\u793a\u6709\u9650\u7684\u975e\u9009\u62e9\u6027\u53cd\u5e94\uff0c\u548c\u76f8\u5bf9\u8f83\u9ad8\u7684\u5149\u8bf1\u5bfc\u6c27\u5316\u6297\u6027\u3002APF\u548cHPF\u5177\u7ec6\u80de\u819c\u6e17\u900f\u6027\uff0c\u672c\u8eab\u65e0\u8367\u5149\uff0c\u76f4\u5230\u4e0e\u7f9f\u57fa\u81ea\u7531\u57fa\u6216\u8fc7\u6c27\u4e9a\u785d\u57fa\u9634\u79bb\u5b50\u53cd\u5e94\uff0c\u8fd8\u80fd\u4e0e\u6b21\u6c2f\u9178\u9634\u79bb\u5b50\u53cd\u5e94\uff0c\u4ea7\u751f\u5f3a\u7eff\u8272\u8367\u5149\uff08Ex\/Em\uff0c~490\/515nm\uff09\uff0c\u53ef\u7528\u8367\u5149\u663e\u5fae\u955c\u3001\u9ad8\u901a\u91cf\u6210\u50cf\u4eea\u3001\u8367\u5149\u9176\u6807\u677f\u6216\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u3002<\/span><\/p>\n

 <\/p>\n

\u672c\u54c1\u4ee5\u7c89\u672b\u5f62\u5f0f\u63d0\u4f9b\uff0c\u53ef\u76f4\u63a5\u6eb6\u4e8eDMF\u914d\u5236\u62105mM\u50a8\u5b58\u6db2\uff0c\u5e38\u7528\u5de5\u4f5c\u6d53\u5ea6\u8303\u56f41-10\u03bcM\uff0c\u9700\u6839\u636e\u81ea\u8eab\u7684\u5b9e\u9a8c\u4f53\u7cfb\u6216\u53c2\u8003\u6587\u732e\u8fdb\u884c\u4f18\u5316\u3002<\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u7279\u6027<\/strong><\/span><\/p>\n

1\uff09CAS NO.\uff1a359010-69-8<\/span><\/p>\n

2\uff09\u540c\u4e49\u540d\uff1a3′-p-(hydroxyphenyl) fluorescein (HPF)\u7f9f\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/p>\n

3\uff09\u5206\u5b50\u5f0f\uff1aC26H16O6<\/span><\/p>\n

4\uff09\u5206\u5b50\u91cf\uff1a424.4<\/span><\/p>\n

5\uff09\u7eaf\u5ea6\uff1a\u226595%<\/span><\/p>\n

6\uff09Ex\/Em\uff1a492\/515nm<\/span><\/p>\n

7\uff09\u6eb6\u89e3\u6027\uff1a\u6eb6\u4e8eDMSO\uff0810mM\uff09\u3001DMF\uff085mM\uff09<\/span><\/p>\n

8\uff09\u5316\u5b66\u7ed3\u6784\uff1a\"\"<\/span><\/p>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/strong><\/span><\/p>\n

\u4fdd\u5b58\uff1a-20\u00baC\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c\u81f3\u5c11\u4e00\u5e74\u6709\u6548\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002\u00a0<\/span><\/p>\n

\u4f7f\u7528\u65b9\u6cd5 <\/strong><\/span><\/p>\n

\u4e00\u3001\u50a8\u5b58\u6db2\u5236\u5907<\/span><\/p>\n

\u5c06\u4f4e\u6e29\u4fdd\u5b58\u76841mg\u7c89\u672b\u7f6e\u4e8e\u5ba4\u6e29\u56de\u6e29\u81f3\u5c1120min\uff0c\u4e4b\u540e\u52a0\u5165471\u03bcl \u65e0\u6c34DMF\u914d\u7f6e\u62105mM\u50a8\u5b58\u6db2\u3002\u5145\u5206\u6eb6\u89e3\u548c\u6df7\u5300\u540e\uff0c\u6309\u7167\u5355\u6b21\u7528\u91cf\u5206\u88c5\uff0c-20\u00baC\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/span><\/p>\n

\u3010\u6ce8\u3011\uff1aHPF\u80fd\u6eb6\u4e8eDMSO\uff0810mM\uff09\uff0c\u4f46\u6709\u6570\u636e\u663e\u793aDMSO\u662f\u7f9f\u57fa\u81ea\u7531\u57fa\u7684\u6dec\u706d\u5242\uff0c\u4f1a\u5e72\u6270\u68c0\u6d4b\u548c\u964d\u4f4e\u7075\u654f\u6027\u3002\u56e0\u6b64\uff0c\u5efa\u8bae\u7528DMF\u4f5c\u4e3a\u6eb6\u5242\u3002<\/span><\/p>\n

\u4e8c\u3001\u6eb6\u6db2\u4f53\u7cfb\u68c0\u6d4b<\/span><\/p>\n

\u6b63\u5f0f\u5b9e\u9a8c\u524d\uff0c\u53d6\u4e00\u7ba1HPF\u50a8\u5b58\u6db2\u7f6e\u4e8e\u5ba4\u6e29\u56de\u6e29\uff0c\u4f7f\u5176\u5145\u5206\u878d\u89e3\u3002\u4e4b\u540e\u7528\u5408\u9002\u7684\u7f13\u51b2\u6db2\uff08\u6bd4\u5982HHBS\u7f13\u51b2\u6db2\uff0cPBS\u7f13\u51b2\u6db2\uff09\u7a00\u91ca\u5230\u6240\u9700\u7684\u5de5\u4f5c\u6d53\u5ea6\u3002\u5efa\u8bae\u8d77\u59cb\u6d53\u5ea6\u4e3a1-10\u03bcM\uff0c\u9700\u6839\u636e\u81ea\u8eab\u7684\u5b9e\u9a8c\u4f53\u7cfb\u6216\u53c2\u8003\u6587\u732e\u8fdb\u884c\u4f18\u5316\u3002<\/span><\/p>\n

\u4e09\u3001\u7ec6\u80de\u4f53\u7cfb\u68c0\u6d4b<\/span><\/p>\n

\u4ee5\u4e0b\u5b9e\u9a8c\u6b65\u9aa4\u4ec5\u505a\u53c2\u8003\uff0c\u6700\u4f73\u7684\u5de5\u4f5c\u6d53\u5ea6\u3001\u5b75\u80b2\u65f6\u95f4\u548c\u6e29\u5ea6\u9700\u6839\u636e\u5b9e\u9645\u60c5\u51b5\u8fdb\u884c\u8c03\u6574\u3002\u5728\u52a0\u5165HPF\u5de5\u4f5c\u6db2\u4e4b\u524d\u6309\u9700\u5904\u7406\u7ec6\u80de\u3002<\/span><\/p>\n

1\uff09\u00a0\u00a0 \u6839\u636e\u5177\u4f53\u7528\u91cf\uff0c\u5c06DMF\u50a8\u5b58\u6db2\u7528HHBS\u7f13\u51b2\u6db2\u6216\u5176\u4ed6\u751f\u7406\u7f13\u51b2\u6db2\u7a00\u91ca\u62101-10\u03bcM\u5de5\u4f5c\u6db2\u3002<\/span><\/p>\n

2\uff09\u00a0\u00a0 \u6839\u636e\u5b9e\u9a8c\u8bbe\u8ba1\u5904\u7406\u7ec6\u80de\uff08\u6bd4\u5982\uff0cRASM\u7ec6\u80de\u752850-100nM \u8840\u7ba1\u7d27\u5f20\u7d20II\u5904\u74063-5h\uff09\u3002<\/span><\/p>\n

3\uff09\u00a0\u00a0 \u53d6\u6b65\u9aa41\uff09\u51c6\u5907\u76841-10\u03bcM\u5de5\u4f5c\u6db2\u52a0\u5165\u7ec6\u80de\uff0c37\u00baC\u5b75\u80b220-60min\u3002<\/span><\/p>\n

4\uff09\u00a0\u00a0 \u5438\u6389\u67d3\u8272\u5de5\u4f5c\u6db2\uff0c\u7528HHBS\u6216\u5176\u4ed6\u751f\u7406\u7f13\u51b2\u6db2\u66ff\u6362\u3002<\/span><\/p>\n

5\uff09\u00a0\u00a0 \u7528\u5408\u9002\u7684\u8367\u5149\u4eea\u5668\uff08Ex\/Em=492\/515nm\uff09\u6765\u68c0\u6d4b\u3002<\/span><\/p>\n

\u3010\u6ce81\u3011\uff1aBSA\u548c\u915a\u7ea2\u4f1a\u5f71\u54cd\u8367\u5149\uff0c\u9700\u8c28\u614e\u4f7f\u7528\u3002<\/span><\/p>\n

\u3010\u6ce82\u3011\uff1aHPF\u548cAPF\u80fd\u7528\u4e8e\u6eb6\u6db2\u4f53\u7cfb\u6216\u80de\u5185ROS\u68c0\u6d4b\u3002<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

1\uff09\u00a0\u00a0 APF\u548cHPF\u4ec5\u7528\u4e8e\u79d1\u7814\u7814\u7a76\uff0c\u4e0d\u53ef\u7528\u4e8e\u4f53\u5916\u8bca\u65ad\u7814\u7a76\u3002<\/span><\/p>\n

2\uff09\u00a0\u00a0 APF\u548cHPF\u4ea7\u751f\u7684\u8367\u5149\u76f8\u5bf9\u4e8e\u5176\u4ed6\u6d3b\u6027\u6c27\u63a2\u9488\u6bd4\u5982DCFH\u5f31\u5f88\u591a\uff0c\u8fd9\u662f\u63a2\u9488\u672c\u8eab\u7684\u7279\u6027\u51b3\u5b9a\u7684\uff08\u89c1\u9644\u88681 \u6d3b\u6027\u6c27\u63a2\u9488\uff08HPF, APF, DCFH\uff09\u5bf9\u5404\u79cd\u6d3b\u6027\u6c27\u7269\u8d28\u7684\u53cd\u5e94\u7279\u5f02\u6027\uff09\u3002<\/span><\/p>\n

3\uff09\u00a0\u00a0 \u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u76f8\u5173\u4ea7\u54c1 <\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n<\/tr>\n
MX4801-1KIT<\/span><\/td>\nReactive Oxygen Species (ROS) Assay Kit \u6d3b \u6027\u6c27\uff08ROS\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n100~500T<\/span><\/td>\n<\/tr>\n
MX4802-50MG<\/span><\/td>\nH2DCFDA (DCFH-DA, DCFH) \u6d3b\u6027\u6c27\uff08ROS\uff09\u8367\u5149\u63a2\u9488<\/span><\/td>\n50mg<\/span><\/td>\n<\/tr>\n
MX4804-1MG<\/span><\/td>\nAminophenyl fluorescein (APF) \u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MX4805-1MG<\/span><\/td>\nHydroxyphenyl fluorescein (HPF) \u7f9f\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MX4455-1MG<\/span><\/td>\nDihydrorhodamine 123 (DHR 123) \u4e8c\u6c22\u7f57\u4e39\u660e123<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MM0706-1G<\/span><\/td>\nLuminol \u9c81\u7c73\u8bfa<\/span><\/td>\n1g<\/span><\/td>\n<\/tr>\n
MX4606-500UG<\/span><\/td>\nCoelenterazine Native \u5929\u7136\u8154\u80a0\u7d20<\/span><\/td>\n500\u03bcg<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u9644\u88681 \u6d3b\u6027\u6c27\u63a2\u9488\uff08HPF, APF, DCFH\uff09\u5bf9\u5404\u79cd\u6d3b\u6027\u6c27\u7269\u8d28\u7684\u53cd\u5e94\u7279\u5f02\u6027 <\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
ROS<\/span><\/td>\nROS\u4ea7\u751f\u65b9\u6cd5<\/span><\/td>\nHPF (MX4805)<\/span><\/td>\nAPF (MX4804)<\/span><\/td>\nDCFH (MX4802)<\/span><\/td>\n<\/tr>\n
HO\u2022<\/span><\/td>\n100 \u00b5M\u9ad8\u6c2f\u9178\u4e9a\u94c1(II)+1 mMH2O2<\/span><\/td>\n730<\/span><\/td>\n1200<\/span><\/td>\n7400<\/span><\/td>\n<\/tr>\n
ONOO\u2013<\/span><\/td>\n3 \u00b5M (\u7ec8\u6d53\u5ea6) ONOO\u2013<\/span><\/td>\n120<\/span><\/td>\n560<\/span><\/td>\n6600<\/span><\/td>\n<\/tr>\n
OCl-<\/span><\/td>\n3 \u00b5M (\u7ec8\u6d53\u5ea6) OCl-<\/span><\/td>\n6<\/span><\/td>\n3600<\/span><\/td>\n86<\/span><\/td>\n<\/tr>\n
1O2<\/span><\/td>\n100 \u00b5M 3-(1,4-dihydro-1,4-epidioxy-1-naphthyl)<\/span><\/p>\n

propionic acid<\/span><\/td>\n

5<\/span><\/td>\n9<\/span><\/td>\n26<\/span><\/td>\n<\/tr>\n
\u2022O2\u2013<\/span><\/td>\n100 \u00b5M KO2<\/span><\/td>\n8<\/span><\/td>\n6<\/span><\/td>\n67<\/span><\/td>\n<\/tr>\n
H2O2<\/span><\/td>\n100 \u00b5M of H2O2<\/span><\/td>\n2<\/span><\/td>\n\uff1c1<\/span><\/td>\n190<\/span><\/td>\n<\/tr>\n
NO<\/span><\/td>\n100 \u00b5M NOC-7<\/span><\/td>\n6<\/span><\/td>\n\uff1c1<\/span><\/td>\n150<\/span><\/td>\n<\/tr>\n
ROO\u2022<\/span><\/td>\n100 \u00b5M AAPH<\/span><\/td>\n17<\/span><\/td>\n2<\/span><\/td>\n710<\/span><\/td>\n<\/tr>\n
Autoxidation<\/span><\/td>\n\u8367\u5149\u5149\u71672.5h<\/span><\/td>\n\uff1c1<\/span><\/td>\n\uff1c1<\/span><\/td>\n2000<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0 <\/strong><\/span><\/p>\n

\u00a0 <\/strong><\/span><\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/span><\/p>\n

\n

\u6587\u732e\u5f15\u7528\uff1a<\/strong><\/span><\/p>\n

[1]Zhen WY\u00a0et\u00a0al.\u00a0Reductive Surfactant-Assisted One-Step Fabrication of BiOI\/BiOIO3 Heterojunction Biophotocatalyst for Enhanced Photodynamic Theranostics Overcoming Tumor Hypoxia<\/u>.<\/u><\/a>\u00a0Nanoscale Horiz., 2019, 4, 720-726<\/span><\/p>\n

Hydroxyphenyl fluorescein (HPF) was purchased from Shanghai Maokang Bio. Co. (Shanghai, China).\u00a0HPF was used to detect the generation of \u2022OH. Typically, 1 mL BB NCs aqueous solution (80 \u00b5g mL -1 ) containing with HPF (1 \u00b5M) were irradiated by 650 nm laser (0.5 W cm-2 ), the fluorescence of HPF was detected after 15 min. HPF aqueous solution with laser served as control.<\/span><\/p>\n

\u00a0\u00a0<\/span><\/p>\n

[2]Liu Yang et al.\u00a0Defect modified zinc oxide with augmenting sonodynamic reactive oxygen species generation.<\/a>\u00a0Biomaterials. Volume 251, August 2020, 120075<\/span><\/p>\n

Hydroxyphenyl\u00a0fluorescein\u00a0(HPF)\u00a0was\u00a0purchased\u00a0from\u00a0Shanghai\u00a0Maokang\u00a0Bio.\u00a0Co. (Shanghai,\u00a0China).<\/span><\/p>\n

[3]Xu X, Huang B, Zeng Z, Chen J, Huang Z, Guan Z, Chen M, Huang Y, Zhao C.\u00a0Broaden sources and reduce expenditure: Tumor-specific transformable oxidative stress nanoamplifier enabling economized photodynamic therapy for reinforced oxidation therapy.\u00a0Theranostics 2020; 10(23):10513-10530. doi:10.7150\/thno.49731.<\/span><\/p>\n

Hydroxyphenyl fluorescein (HPF) was purchased from Shanghai Maokang Bio. Co. (Shanghai, China).\u00a0For detecting intracellular \u2022OH generation, treated B16F10 cells were rinsed and stained with specific \u2022OH probe HPF (10 \u03bcM in PBS) for 60 min, followed by rinsed with PBS and imaged using CLSM. To further confirm the Fe(II) consumption, after treatment with H2O2 (positive control), CA and HA@CA for 12 h, the cells were stained with a ferric ion probe, 3\u2032,6\u2032-bis (diethylamino)-2-(4-oxopent-2-en-2-ylamino) spiro (isoindoline-1,9\u2032-xanthen)-3-one (10 \u03bcM) at 37 \u00b0C for 30 min. Finally, the treated cells were rinsed with PBS and observed by CLSM.<\/span><\/p>\n

[4]Tang H, Li C, Zhang Y, et al.\u00a0Targeted Manganese doped silica nano GSH-cleaner for treatment of Liver Cancer by destroying the intracellular redox homeostasis.<\/a>\u00a0Theranostics. 2020;10(21):9865-9887. Published 2020 Aug 2. doi:10.7150\/thno.46771<\/span><\/p>\n

Hydroxyphenyl fluorescein (HPF) was supplied by Shanghai Mao Kang biotechnology Co., Ltd.\u00a0For evaluating the different types of ROS, DHE and HPF were selected as probes to assess the generation of intracellular \u2022O2- and \u2022OH, respectively. Briefly, HepG2 cells (5\u00d7105 cells per well) were seeded in a 6-well plate and incubated overnight. After that, Free SFB, MnMSN@SFB or FaPEG-MnMSN@SFB at different concentrations (10, 20, and 40 \u00b5g\/mL of SFB equivalent) were added and treated for 6 h. Cells were then stained with DHE (20 \u00b5M) for another 45 min. Eventually, fluorescence microscope was employed to observe the fluorescence of cells at the wavelength of 535 nm. Moreover, to assess the generation of intracellular \u2022OH, HepG2 cells (1\u00d7105 cells per well) were seeded into confocal dishes and incubated for 12 h. Then, Free SFB, MnMSN@SFB or FaPEG-MnMSN@SFB at different concentrations (10, 20, and 40 \u03bcg\/mL of SFB equivalent) were added, meanwhile HPF (10 \u00b5M) was added to capture generative intracellular \u2022OH, following by co-incubation for 6 h. Eventually, CLSM was used to observe the fluorescence of cells at 490 nm.<\/span><\/p>\n

\u30105\u3011<\/span><\/p>\n

Lili Zhang, Jiabao Huang, Shiqi Su, Xiaochun Wei, Lin Yang, Huanhuan Zhao, Jianqiang Yu, Jie Wang, Jiyun Hui, Shiya Hao, Shanshan Song, Yanyan Cao, Maoshuai Wang, Xiaowei Zhang, Yanyan Zhao, Zhiyong Wang, Weiqing Zeng, Hen-Ming Wu, Yuxiang Yuan, Xiansheng Zhang, Alice Y. Cheung, Qiaohong Duan,<\/p>\n

FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa, Current Biology, 2021, ISSN 0960-9822,\u00a0https:\/\/doi.org\/10.1016\/j.cub.2021.04.060<\/a>.<\/p>\n

Probe 20,70-Dichlorodihydrofluorescein diacetate (H2DCFDA), dihydroethidium (DHE), hydroxyphenyl fluorescein (HPF) are commonly used for ROS detection.\u00a0HPF was used at 10 mM in 0.2 M PBS buffer (pH 6.1) for 2 hours. Comparable results were obtained in experiments tested with all three dyes, providing confidence for the observed changes in ROS levels.<\/p>\n

\n

\u30106\u3011<\/p>\n

Mengsi Wu,\u00a0Zhiyong Liu,\u00a0Weian Zhang.\u00a0An ultra-stable bio-inspired bacteriochlorin analogue for hypoxia-tolerant photodynamic therapy.\u00a0Chemical Science\u00a02021,\u00a012\u00a0(4) , 1295-1301.\u00a0https:\/\/doi.org\/10.1039\/D0SC05525E<\/a><\/p>\n

Singlet oxygen sensor green (SOSG), dihydroethidium (DHE), 3\u2019-(4-hydroxyphenyl) fluorescein (HPF), Fetal bovine serum (FBS), Dulbecco\u2019s modified Eagle\u2019s medium (DMEM), Hoechst 33342 and 3-(4,5- dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Shanghai Maokang Biotechnology Co.,Ltd.\u00a0 Hydroxyl radical (\u2022OH) was detected by fluorescence spectroscopy using hydroxyphenyl fluorescein (HPF), which reacts with \u2022OH to produce high fluorescence emission at 530 nm. HPF was dissolved in DMF to gain 5 mM HPF solution. Then, 20 \u03bcL HPF was added into the solution of 2 mL FBC or FBC nanogels. The fluorescence changes were detected after illumination with 750 nm laser, and the excitation wavelength was 490 nm.<\/p>\n

\n

\u30107\u3011<\/p>\n

Jinghua An, Xiaoting Song, Wenbo Wan, Yanzheng Chen, Haibin Si, Huichuan Duan, Lu Li*, and Bo Taing*. Kinetics of the Photoelectron-Transfer Process Characterized by Real-Time Single-Molecule Fluorescence Imaging on Individual Photocatalyst Particles. ACS Catal. 2021, 11, 12, 6872\u20136882 Publication Date:May 27, 2021\u00a0https:\/\/doi.org\/10.1021\/acscatal.1c00983<\/a><\/p>\n

HPF and Amplex red from maokang biotechnology co. ltd.<\/p>\n","protected":false},"excerpt":{"rendered":"

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