{"id":29192,"date":"2021-07-30T15:59:31","date_gmt":"2021-07-30T07:59:31","guid":{"rendered":"\/\/www.562part.com\/?post_type=product&p=29192"},"modified":"2022-08-02T11:21:31","modified_gmt":"2022-08-02T03:21:31","slug":"zeocin-100mg-ml-%e5%8d%9a%e8%8e%b1%e9%9c%89%e7%b4%a0%ef%bc%88100-mg-ml%ef%bc%89-4","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/zeocin-100mg-ml-%e5%8d%9a%e8%8e%b1%e9%9c%89%e7%b4%a0%ef%bc%88100-mg-ml%ef%bc%89-4\/","title":{"rendered":"Zeocin (100mg\/ml) \u535a\u83b1\u9709\u7d20\uff08100 mg\/ml\uff09"},"content":{"rendered":"

\"\"\u00a0<\/span><\/span><\/p>\n

 <\/p>\n

\u3010\u6e29\u99a8\u63d0\u793a\u3011\uff1a\u5982\u679c\u4f60\u5bf9\u5e02\u573a\u4e0a\u5e38\u89c1\u4e2d\u6587\u540d\u90fd\u53eb\u3010\u535a\u83b1\u9709\u7d20\u3011\uff0c\u4f46\u82f1\u6587\u540d\u53c8\u4e0d\u540c<\/u>\u7684\u4e00\u4e9b\u4ea7\u54c1\u611f\u5230\u6709\u6240\u8ff7\u60d1\uff0c\u53ef\u53c2\u8003\u6211\u53f8\u3010\u61cb\u5eb7\u751f\u7269\u3011\u6574\u7406\u7684Bleomycins (BLMs) \u535a\u83b1\u9709\u7d20\u5bb6\u65cf\u5e38\u7528\u6297\u751f\u7d20\u6210\u5458\u7684\u5e94\u7528\u9009\u62e9<\/a><\/u>\uff0c\u4e5f\u8bb8\u6709\u6240\u6536\u83b7\u3002<\/strong><\/span><\/p>\n

\u535a\u83b1\u9709\u7d20\u5bb6\u65cf\u5e38\u89c1\u4ea7\u54c1\uff1a<\/strong><\/span><\/p>\n

\u7b5b\u9009\u6297\u751f\u7d20\uff1aZeocin\u3010\u51bb\u5e72\u7c89\u3011\u00a0<\/a>\u00a0Zeocin\u3010100mg\/ml<\/a>\u3011\u00a0\u00a0\u00a0<\/a>\u00a0\u00a0Phleomycin\u00a0\u8150\u8349\u9709\u7d20<\/a><\/strong><\/span><\/p>\n

\u7ec6\u80de\u529f\u80fd\u7814\u7a76\uff1aBleomycin\u00a0Sulfate \u786b\u9178\u535a\u83b1\u9709\u7d20<\/a>\u00a0\u00a0\u00a0\u00a0\u00a0Bleomycin A5 Hydrochloride \u76d0\u9178\u535a\u83b1\u9709\u7d20A5<\/a><\/strong><\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u5173\u952e\u8bcd\uff1a<\/strong><\/span><\/p>\n

Zeocin \u535a\u83b1\u9709\u7d20\uff08\u535a\u6765\u9709\u7d20\uff09\uff1bPhleomycin \u8150\u8349\u9709\u7d20\uff1bSh ble\u57fa\u56e0\uff1bStreptomyces verticillus \u8f6e\u679d\u94fe\u9709\u83cc\uff1bHygromycin B \u6f6e\u9709\u7d20B\uff1bG418\u9057\u4f20\u9709\u7d20\uff1bCAS NO.\uff1a11006-33-0<\/span><\/p>\n

\u57fa\u672c\u63cf\u8ff0\uff1a<\/strong><\/span><\/p>\n

Zeocin\u2122\u662f\u535a\u6765\u9709\u7d20\/\u8150\u8349\u9709\u7d20\uff08bleomycin\/phleomycin\uff09\u6297\u751f\u7d20\u5bb6\u65cf\u7684\u6210\u5458\u4e4b\u4e00\uff0c\u4ece\u8f6e\u679d\u94fe\u9709\u83ccStreptomyces verticillus\u4e2d\u5206\u79bb\u5f97\u5230\uff0c\u5bf9\u7ec6\u83cc\u3001\u771f\u83cc\uff08\u5305\u62ec\u9175\u6bcd\u83cc\uff09\u3001\u690d\u7269\u548c\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u5177\u5f88\u5f3a\u7684\u6bd2\u6027\u3002\u6765\u81eaStreptoalloteichus hindustanus\u7684Sh ble\u57fa\u56e0\u8d4b\u4e88Zeocin\u6297\u6027\u3002<\/span><\/p>\n

Zeocin\u2122\u662f\u8150\u8349\u9709\u7d20D1\u7684\u5546\u4e1a\u540d\u79f0\uff0c\u4e00\u79cd\u78b1\u6027\u3001\u6c34\u6eb6\u6027\u3001\u94dc\u79bb\u5b50\u87af\u5408\u7684\u7cd6\u80bd\u6297\u751f\u7d20\u3002\u94dc\u79bb\u5b50\u7684\u5b58\u5728\u4f7f\u5f97\u6eb6\u6db2\u4e3a\u84dd\u8272\uff0c\u6b64\u94dc\u87af\u5408\u7684\u5f62\u5f0f\u4e3a\u65e0\u6d3b\u6027\u3002\u4e00\u65e6\u8fdb\u5165\u7ec6\u80de\u540e\uff0c\u4e8c\u4ef7\u94dc\u79bb\u5b50\uff08Cu2+\uff09\u88ab\u8fd8\u539f\u4e3a\u4e00\u4ef7\u94dc\u79bb\u5b50\uff08Cu+\uff09\uff0c\u5e76\u88ab\u7ec6\u80de\u5185\u7684\u5def\u57fa\u5316\u5408\u7269\u53bb\u9664\u3002\u94dc\u79bb\u5b50\u88ab\u53bb\u9664\u540e\uff0cZeocin\u2122\u88ab\u6d3b\u5316\uff0c\u5d4c\u5165DNA\u4f7f\u5176\u65ad\u88c2\uff0c\u6700\u7ec8\u5bfc\u81f4\u7ec6\u80de\u6b7b\u4ea1\u3002<\/span><\/p>\n

\u6297\u6027\u57fa\u56e0\uff1a<\/strong><\/span><\/p>\n

Zeocin\u2122\u6297\u6027\u7531\u7f16\u7801\u4e00\u79cd13,665Da\u5927\u5c0f\u86cb\u767d\u7684Sh ble\u57fa\u56e0\uff08Streptoalloteichus hindustanus bleomycin gene\uff09\u8d4b\u4e88\uff0c\u8be5\u86cb\u767d\u5316\u5b66\u8ba1\u91cf\u65b9\u5f0f\u7ed3\u5408Zeocin\u2122\uff0c\u6291\u5236\u5176DNA\u53cc\u94fe\u5207\u5272\u6d3b\u6027\u3002\u56e0\u6b64\uff0c\u80fd\u8868\u8fbe\u6b64\u86cb\u767d\u7684\u771f\u6838\u548c\u539f\u6838\u5bbf\u4e3b\u7ec6\u80de\u88ab\u8d4b\u4e88Zeocin\u2122\u6297\u6027\u3002<\/span><\/p>\n

\u57fa\u672c\u7279\u6027\uff1a<\/strong><\/span><\/p>\n

1\uff09CAS NO.\uff1a11006-33-0<\/span><\/p>\n

2\uff09\u5316\u5b66\u5f0f\uff1aC55H83N19O21S2Cu<\/span><\/p>\n

3\uff09\u5206\u5b50\u91cf\uff1a1137.41 g\/mol<\/span><\/p>\n

4\uff09\u5916\u89c2\uff1a\u84dd\u8272\u6eb6\u6db2\uff08100mg\/ml in deionized\uff0cautoclaved water\uff09<\/span><\/p>\n

5\uff09\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93<\/span><\/p>\n

6\uff09\u4fdd\u5b58\uff1a-30\u00b0C~-10\u00b0C\uff0c\u81f3\u5c111\u5e74\u6709\u6548<\/span><\/p>\n

7\uff09\u5927\u80a0\u6746\u83cc\u7b5b\u9009\uff1a25-50\u03bcg\/mL\uff08\u4f4e\u76d0LB\u57f9\u517b\u57fa\uff0cNaCl\u6d53\u5ea6\u4e0d\u80fd\u8d85\u8fc75g\/L\uff09<\/span><\/p>\n

8\uff09\u9175\u6bcd\u83cc\u7b5b\u9009\uff1a50-300\u03bcg\/mL\uff08YPD\u6216\u57fa\u672c\u57f9\u517b\u57fa\uff09<\/span><\/p>\n

9\uff09\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u7b5b\u9009\uff1a50-1000\u03bcg\/mL\uff08\u5408\u9002\u57f9\u517b\u57fa\uff0c\u6839\u636e\u7ec6\u80de\u7cfb\u800c\u4e0d\u540c\uff09<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879\uff1a<\/strong><\/span><\/p>\n

1\uff09Zeocin\u2122\u5bf9\u5149\u654f\u611f\uff0c\u9700\u5c06\u6297\u751f\u7d20\uff0c\u6216\u542b\u8be5\u6297\u751f\u7d20\u7684\u5e73\u677f\u6216\u57f9\u517b\u57fa\u7f6e\u4e8e\u9ed1\u6697\u5904\u3002<\/span><\/p>\n

2\uff09\u964d\u4f4e\u7ec6\u83cc\u57f9\u517b\u57fa\u7684\u76d0\u6d53\u5ea6\uff0c\u8c03\u6574pH\u52307.5\u4f7f\u5176\u4fdd\u6301\u6d3b\u6027\uff0c\u56e0\u9ad8\u79bb\u5b50\u5f3a\u5ea6\u548c\u9178\u6216\u78b1\u6027\u90fd\u4f1a\u6291\u5236Zeocin\u2122\u6d3b\u6027\uff1b<\/span><\/p>\n

3\uff09\u50a8\u5b58Zeocin\u2122\u5728-30\u00b0C~-10\u00b0C\uff0c\u4f7f\u7528\u524d\u9700\u51b0\u4e0a\u878d\u5316\u3002<\/span><\/p>\n

4\uff09Zeocin\u2122\u6709\u6bd2\uff0c\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u4f7f\u7528\u65b9\u6cd5\uff08\u5e94\u7528\u4e3e\u4f8b\uff09\uff1a<\/strong><\/span><\/p>\n

1\uff09\u5927\u80a0\u6746\u83cc\uff08E. Coli.\uff09<\/span><\/p>\n

\u5bbf\u4e3b\uff1a\u4e0d\u80fd\u5305\u542bTn5\u8f6c\u5ea7\u5b50\uff08\u6bd4\u5982\uff1aTop10\uff0cDH5\uff0cDH10\uff09<\/span><\/p>\n

\u57f9\u517b\u57fa\uff1a\u4f7f\u7528\u4f4e\u76d0LB\uff0810g Trypton\uff0c5g NaCl\uff0c5g Yeast extract\uff09\uff0cpH 7.5\uff0c\u9632\u6b62Zeocin\u2122\u5931\u6d3b<\/span><\/p>\n

\u9009\u62e9\u6d53\u5ea6\uff1a25-50\u03bcg\/mL\u7528\u4e8e\u5927\u80a0\u6746\u83cc\u7b5b\u9009<\/span><\/p>\n

2\uff09\u9175\u6bcd\u83cc\uff08Yeast\uff09<\/span><\/p>\n

\u5bbf\u4e3b\uff1a\u917f\u9152\u9175\u6bcd\uff08Saccharomyces cerevisiae\uff09,\u6bd5\u8d64\u9175\u6bcd\uff08Pichia pastoris\uff09<\/span><\/p>\n

\u57f9\u517b\u57fa\uff1a\u542b1M\u5c71\u68a8\u9187\u7684YPD\uff08\u7535\u8f6c\u5316\u7ec6\u80de\uff09\uff1bYPD\u6216\u57fa\u672c\u57f9\u517b\u57fa\uff08\u5316\u5b66\u8f6c\u5316\u7ec6\u80de\uff09\uff1b\u6d4b\u8bd5\u57f9\u517b\u57fa\u8c03pH\u52306.5-8.0\uff0c\u9009\u62e9pH\u503c\u5141\u8bb8\u4f7f\u7528\u6700\u4f4e\u6d53\u5ea6Zeocin\u2122\u3002<\/span><\/p>\n

\u8f6c\u5316\u65b9\u6cd5\uff1a\u7535\u8f6c\u6cd5\uff0c\u4f7f\u7528\u9502\u79bb\u5b50\u6b65\u9aa4\u6216\u4f7f\u7528EasyComp\u8bd5\u5242\u76d2\u3002\u4e0d\u80fd\u4f7f\u7528\u539f\u751f\u8d28\u4f53\u7528\u4e8e\u9175\u6bcd\u8f6c\u5316\uff0c\u56e0Zeocin\u2122\u4f1a\u5f15\u8d77\u5b8c\u6574\u7ec6\u80de\u6b7b\u4ea1\u3002<\/span><\/p>\n

\u9009\u62e9\u6d53\u5ea6\uff1a50-300\u03bcg\/mL\uff0c\u53d6\u51b3\u4e8e\u9175\u6bcd\u83cc\u7c7b\u578b\u3001\u57f9\u517b\u57fapH\u548c\u79bb\u5b50\u5f3a\u5ea6\u3002\u5efa\u7acb\u6740\u706d\u66f2\u7ebf\u786e\u5b9a\u80fd\u6740\u6b7b\u672a\u8f6c\u5316\u5bbf\u4e3b\u7ec6\u80de\u7684\u6700\u4f4eZeocin\u2122\u6d53\u5ea6\u3002<\/span><\/p>\n

3\uff09\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\uff08Mammalian cells\uff09<\/span><\/p>\n

50-1000\u03bcg\/mL\u7528\u4e8e\u7b5b\u9009\u7a33\u5b9a\u8f6c\u67d3\u7ec6\u80de\u7cfb\uff08\u5e73\u5747\u5e38\u7528\u6d53\u5ea6\u4e3a250-400\u03bcg\/mL\uff09\u3002\u53d6\u51b3\u4e8e\u7ec6\u80de\u7cfb\uff0c\u9700\u89812-6\u5468\u7528Zeocin\u2122\u7b5b\u9009\u5f97\u5230\u7a33\u8f6c\u96c6\u843d\uff08foci\uff09\u3002\u8fdb\u884c\u7a33\u8f6c\u682a\u6b63\u5f0f\u7b5b\u9009\u524d\uff0c\u9700\u786e\u5b9a\u80fd\u591f\u6740\u6b7b\u6240\u6709\u672a\u8f6c\u67d3\u5bbf\u4e3b\u7ec6\u80de\u7684\u6700\u4f4eZeocin\u2122\u6d53\u5ea6\u3002<\/span><\/p>\n

1\uff09 \u786e\u5b9a\u7ec6\u80de\u5bf9Zeocin\u2122\u7684\u654f\u611f\u6027<\/span><\/p>\n

2\uff09\u7b5b\u9009\u7a33\u5b9a\u8f6c\u67d3\u7ec6\u80de\u7cfb<\/span><\/p>\n

\u7b5b\u9009\u5c0f\u6280\u5de7\uff1a\u82e5\u662f\u7ec6\u80de\u5bf9Zeocin\u2122\u8010\u6027\u66f4\u5f3a\uff0c\u53ef\u7528\u542bZeocin\u2122\u57f9\u517b\u57fa\u8fdb\u884c\u7ec6\u80de\u5206\u76d8\uff0c\u5e76\u7f6e\u4e8e37\u00b0C\u5b75\u80b22-3h\uff0c\u4f7f\u5f97\u7ec6\u80de\u8d34\u58c1\u3002\u7136\u540e\u5c06\u7ec6\u80de\u7f6e\u4e8e4\u00b0C\uff0c2h\u3002\u8bb0\u5f97\u4f7f\u7528Hepes\u4f5c\u4e3a\u57f9\u517b\u57fa\u7684\u7f13\u51b2\u4f53\u7cfb\u3002\u91cd\u65b0\u5c06\u7ec6\u80de\u7f6e\u4e8e37\u00b0C\u5b75\u80b2\u30024\u00b0C\u5b75\u80b2\u7ec6\u80de\u7684\u76ee\u7684\u662f\u77ed\u65f6\u95f4\u5185\u7ec8\u6b62\u7ec6\u80de\u5206\u5316\uff0c\u4f7f\u5f97Zeocin\u2122\u80fd\u53d1\u6325\u4f5c\u7528\uff0c\u6740\u6b7b\u7ec6\u80de\u3002<\/span><\/p>\n

\u8ba2\u8d2d\u4fe1\u606f\uff1a\u539f\u88c5\u8fdb\u53e3\uff0c\u73b0\u8d27\u4f9b\u5e94\u3002<\/strong>\u6b22\u8fce\u6765\u7535\u8ba2\u8d2d\uff0c\u7535\u8bdd\uff1a021-54736159\uff0c\u624b\u673a\uff1a18121428569\uff0c\u6768\u5148\u751f\u3002<\/span><\/p>\n\n\n\n\n\n
\u4ea7\u5730\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u4ea7\u54c1\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u4ea7\u54c1\u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u89c4\u683c\u00a0<\/span><\/td>\n\u00a0\u5355\u4f4d\u00a0<\/span><\/td>\n\u00a0\u4fc3\u9500\u4ef7\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
Invitrogen<\/span><\/td>\nZeocin\u00a0\u535a\u83b1\u9709\u7d20\uff08100mg\/ml\uff09<\/span><\/td>\nR250-01<\/span><\/td>\n125mg\uff081.25ml\uff09<\/span><\/td>\n\u652f<\/span><\/td>\n550<\/span><\/td>\n<\/tr>\n
Invitrogen<\/span><\/td>\nZeocin\u00a0\u535a\u83b1\u9709\u7d20\uff08100mg\/ml\uff09<\/span><\/td>\nR250-01<\/span><\/td>\n1g\uff088 x 1.25ml\uff09<\/span><\/td>\n\u76d2<\/span><\/td>\n3250<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3010\u6ce8\u610f1\u3011\uff1a<\/strong>\u5bf9Zeocin\u4e0d\u654f\u611f\u7684\u7ec6\u80de\uff0c\u5982\u4e1d\u72b6\u771f\u83cc\u548c\u4e00\u4e9b\u9175\u6bcd\u83cc\uff0c\u5efa\u8bae\u4f7f\u7528\u8150\u8349\u9709\u7d20\uff08\u8d27\u53f7\uff1aMS0008-1ML\uff09\uff0c<\/a><\/strong><\/u>\u5176\u6297\u6027\u540c\u6837\u6e90\u4e8eSh ble\u57fa\u56e0\u3002\u00a0\u3010\u6ce8\u610f2\u3011\uff1a\u7279\u4ef7\u4f9b\u5e94\u542bZeo+\u57fa\u56e0\u5e8f\u5217\u7684\u8d28\u7c92\u2014 \u2014\u00a0pcDNA 3.1\/Zeo\uff08+\uff09\uff08Invitrogen of Thermo\uff09<\/a>\uff0c\u5e8f\u5217\u56fe\u8c31\u70b9\u51fb\u4e0b\u8f7d\u6587\u6863\u3002<\/span><\/p>\n

\u5e94\u7528\u56fe\u7247\u3010Zeocin\u2122\u7b5b\u9009\u4e0b\u7684HEK293\u7ec6\u80de\u3011\uff1a<\/strong><\/span><\/p>\n

HEK293\u7ec6\u80de\u7528\u542b10% FBS\uff0c1mM L-\u8c37\u6c28\u9170\u80fa\u548c400\u03bcg\/mL\u53cc\u6297\u7684\u57f9\u517b\u57fa\uff0c\u5206\u522b\u5728\u6dfb\u52a0\u548c\u672a\u6dfb\u52a0400\u03bcg\/mLZeocin\u2122\u7684\u57f9\u517b\u57fa\u9009\u62e9\u57f9\u517b\u3002<\/span><\/p>\n

\u00a0\u56fe1\uff0c\u672a\u7ecfZeocin\u2122\u7b5b\u9009\u7684HEK293\u7ec6\u80de\u3002<\/span><\/p>\n

\u00a0\u56fe2\uff0c\u7ecfZeocin\u2122\u7b5b\u90093\u5929\u540e\u7684HEK293\u7ec6\u80de\u3002\u968f\u7740\u8d28\u819c\u7834\u88c2\uff0c\u5f80\u7ec6\u80de\u5916\u5ef6\u4f38\u957f\u51fa\u957f\u81c2\uff08long appendages\uff09\u3002\uff08\u89c1\u5b9e\u5fc3\u7bad\u5934\u6807\u6ce8\uff09<\/span><\/p>\n

\u00a0\u56fe3\uff0c\u7ecfZeocin\u2122\u7b5b\u90093\u5929\u540e\u7684HEK293\u7ec6\u80de\u3002\u7ec6\u80de\u5f00\u59cb\u7834\u88c2\uff0c\u57f9\u517b\u57fa\u5185\u53d1\u73b0\u7ec6\u80de\u9897\u7c92\u3002\uff08\u89c1\u7a7a\u5fc3\u7bad\u5934\u6807\u6ce8\uff09<\/span><\/p>\n

\n

\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52j\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u66f4\u591a\u4ea7\u54c1\u4fe1\u606f\u8bf7\u54a8\u8be2\u5ba2\u670d\uff01<\/p>\n","protected":false},"featured_media":29193,"template":"","meta":[],"product_cat":[15],"product_tag":[102],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product\/29192"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media\/29193"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=29192"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_cat?post=29192"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_tag?post=29192"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}