{"id":17644,"date":"2019-09-03T16:35:08","date_gmt":"2019-09-03T08:35:08","guid":{"rendered":"\/\/www.562part.com\/?post_type=product&p=17644"},"modified":"2023-04-26T14:33:41","modified_gmt":"2023-04-26T06:33:41","slug":"mitotracker-orange-cmtmros","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/mitotracker-orange-cmtmros\/","title":{"rendered":"MitoTracker\u2122 Orange CMTMRos \u6a59\u8272\u7ebf\u7c92\u4f53\u8367\u5149\u63a2\u9488"},"content":{"rendered":"

MitoTracker\u2122 Orange CMTMRos<\/strong><\/span><\/p>\n

\u6a59\u8272\u7ebf\u7c92\u4f53\u8367\u5149\u63a2\u9488<\/strong><\/span><\/p>\n

 <\/p>\n

\u8ba2\u8d2d\u4fe1\u606f\uff1a<\/strong><\/span><\/p>\n\n\n\n\n
\n

\u8d27\u53f7<\/span><\/p>\n<\/td>\n

\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n\u4fc3\u9500\u4ef7\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
M7510<\/span><\/td>\nMitoTracker\u00ae Orange CMTMRos\u00a0\u6a59\u8272<\/span><\/td>\n50 \u00b5g<\/span><\/td>\n\n

450<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ee5\u4e0a\u6240\u6709\u4ea7\u54c1\u5747\u4e3aLife\uff08Invitrogen\uff09\u539f\u88c5\u6b63\u54c1\uff0c\u539f\u59cb\u5305\u88c5\u4e3a20×50 \u00b5g\uff0820\u652f50\u00b5g\u5355\u4e2a\u5305\u88c5\u6784\u6210\uff09\u3002\u6211\u53f8\u5bf9M7510\u8fdb\u884c\u62c6\u5f00\u9500\u552e\uff0c\u7279\u60e0\u4ef7\u4e3a450\u5143\/\u652f\uff0c\u5355\u652f50\u00b5g\uff0c\u73b0\u8d27\u9500\u552e\uff0c\u6b22\u8fce\u8ba2\u8d2d\u3002<\/span><\/p>\n

 <\/p>\n

\u57fa\u672c\u4ecb\u7ecd\uff1a<\/strong><\/span><\/p>\n

MitoTracker\u00ae Orange CMTMRos\uff08M7510\uff09\u662f\u4e00\u79cd\u6a59\u8272\u8367\u5149\u67d3\u6599\uff0c\u7528\u4e8e\u5bf9\u6d3b\u7ec6\u80de\u7ebf\u7c92\u4f53\u8fdb\u884c\u67d3\u8272\uff0c\u8be5\u67d3\u6599\u7684\u79ef\u7d2f\u53d6\u51b3\u4e8e\u819c\u7535\u4f4d\u3002\u4e59\u919b\u56fa\u5b9a\u540e\uff0c\u8be5\u67d3\u6599\u53ef\u7a33\u5b9a\u4fdd\u5b58\u3002<\/span><\/p>\n

\u4ea7\u54c1\u7279\u6027<\/strong><\/span><\/p>\n

1\uff09\u00a0CAS NO.\uff1aN\/A<\/span><\/p>\n

2\uff09\u00a0\u5206\u5b50\u5f0f\uff1aC24H24Cl2N2O<\/span><\/p>\n

3\uff09\u00a0\u5206\u5b50\u91cf\uff1a427.37<\/span><\/p>\n

4\uff09\u00a0\u5916\u89c2\uff1a\u7d2b\u8272\u56fa\u4f53\uff08purple solid\uff09<\/span><\/p>\n

5\uff09\u00a0\u6fc0\u53d1\/\u53d1\u5c04\uff1a554\/576nm<\/span><\/p>\n

6\uff09\u00a0\u7ed3\u6784\u5f0f\uff1a\"\"<\/span><\/p>\n

7\uff09\u00a0\u6eb6\u89e3\u6027\uff1aDMSO\uff081mM\uff09<\/span><\/p>\n

 <\/p>\n

\u67d3\u8272\u793a\u4f8b<\/strong><\/span><\/p>\n

\"\"<\/span><\/p>\n

Fig 1.A549 spheroid mounted with Prolong Glass and imaged with confocal microscope with lateral and axial projections.A549 spheroid grown in Nunclon Sphera microplates (Cat. No. 174925) at 1000 cells\/well. Live cells were labeled with NucBlue Live ReadyProbes Reagent (Cat. No. R37605), MitoTracker Orange (Cat. No. M7510), and CellROX Deep Red Reagent for oxidative stress detection (Cat. No. C10422) for 1 hour at 37C. Cells were then fixed with 4% PFA, transferred to a #1.5 coverslip, and mounted in ProLong Glass. Sample was allowed to cure overnight exposed to air, and the coverslip was mounted to the slide using 100% glycerol. Image acquisition and analysis was performed using a Zeiss LSM710 Confocal running Zen software.<\/span><\/p>\n

 <\/p>\n

MitoTracker Probes\u7cfb\u5217\u63a2\u9488\u4e13\u9898\uff1a<\/span><\/strong><\/p>\n

1\uff09\u00a0\u4f20\u7edf\u7ebf\u7c92\u4f53\u63a2\u9488\u7684\u7f3a\u70b9\uff1a<\/span><\/p>\n

\u4f20\u7edf\u7ebf\u7c92\u4f53\u63a2\u9488\u5982\u7f57\u4e39\u660e123\uff08Rhodamine 123\uff09\u867d\u7136\u53ef\u7acb\u5373\u805a\u96c6\u5728\u529f\u80fd\u7ebf\u7c92\u4f53\u4e0a\uff0c\u7136\u800c\uff0c\u4e00\u65e6\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\u4e27\u5931\uff0c\u8fd9\u4e9b\u63a2\u9488\u5c31\u80fd\u5f88\u5bb9\u6613\u6e05\u6d17\u6389\u79bb\u5f00\u7ec6\u80de\u3002\u5f53\u78b0\u5230\u7ec6\u80de\u9700\u8981\u8fdb\u884c\u919b\u7c7b\u56fa\u5b9a\u6216\u7ec6\u80de\u5185\u542b\u5f71\u54cd\u819c\u7535\u4f4d\u836f\u7269\u7684\u5b9e\u9a8c\u4e2d\uff0c\u8fd9\u4e9b\u63a2\u9488\u5219\u53d7\u5230\u9650\u5236\uff0c\u4e0d\u53ef\u4f7f\u7528\u3002<\/span><\/p>\n

2\uff09\u00a0MitoTracker\u7ebf\u7c92\u4f53\u63a2\u9488\u7684\u4f18\u70b9<\/span><\/p>\n

Mitotracker\u6b63\u514b\u670d\u4f20\u7edf\u63a2\u9488\u7684\u7f3a\u70b9\u800c\u88ab\u751f\u4ea7\uff0c\u5176\u80fd\u591f\u805a\u96c6\u5728\u6d3b\u7ebf\u7c92\u4f53\u4e0a\uff0c\u5e76\u4e14\u7ec6\u80de\u56fa\u5b9a\u4e2d\u4e5f\u80fd\u826f\u597d\u7ef4\u6301\u3002\u5176\u4e2d\uff0cMitotracker orange\uff0cred\uff0cand deep red\u8fd9\u4e09\u79cd\u63a2\u9488\u7ecf\u900f\u5316\uff08permeabilization\uff09\u540e\u4ecd\u80fd\u826f\u597d\u7ef4\u6301\u3002\u5728\u63a5\u4e0b\u6765\u514d\u75ab\u7ec6\u80de\u5316\u5b66\uff08ICC\uff09\u3001\u539f\u4f4d\u6742\u4ea4\uff08ISH\uff09\u6216\u7535\u955c\u5b9e\u9a8c\u7684\u5904\u7406\u6b65\u9aa4\u4e2d\u6837\u672c\u4ecd\u4fdd\u7559\u7ebf\u7c92\u4f53\u7684\u6d3b\u7ec6\u80de\u67d3\u8272\u5f62\u6001\u3002\u53e6\u5916\uff0cMitotracker\u63a2\u9488\u53bb\u9664\u75c5\u539f\u7ec6\u80de\u7ebf\u7c92\u4f53\u67d3\u8272\u53ef\u80fd\u9047\u5230\u7684\u4e00\u4e9b\u95ee\u9898\uff0c\u56e0\u4e3a\u4e00\u65e6\u7ebf\u7c92\u4f53\u67d3\u8272\u540e\uff0c\u7ec6\u80de\u5373\u53ef\u88ab\u56fa\u5b9a\u7136\u540e\u8fdb\u884c\u7ed3\u679c\u5206\u6790\u3002<\/span><\/p>\n

3\uff09\u00a0MitoTracker\u7ebf\u7c92\u4f53\u63a2\u9488\u4ea7\u54c1<\/span><\/p>\n

\u6211\u4eec\u63d0\u4f9b7\u79cdMitotracker\u63a2\u9488\uff0c\u5b83\u4eec\u5728\u5149\u8c31\u7279\u6027\u3001\u6c27\u5316\u6001\u4ee5\u53ca\u53ef\u5426\u56fa\u5b9a\u51e0\u4e2a\u53c2\u6570\u4e0a\u6709\u6240\u5dee\u5f02\uff0c\u5177\u4f53\u89c1\u4e0b\u8868\u3002<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\nEx\/Em(nm)<\/span><\/td>\n\u662f\u5426\u53ef\u4ee5\u56fa\u5b9a<\/span><\/td>\n\u6c27\u5316\u72b6\u6001<\/span><\/td>\n<\/tr>\n
Rosamine-based MitoTracker Probes<\/span><\/td>\n<\/tr>\n
M7510<\/span><\/td>\nMitoTracker\u00ae Orange CMTMRos\u00a0\u6a59\u8272<\/span><\/td>\n554\u2044576<\/span><\/td>\nYes<\/span><\/td>\nOxidized<\/span><\/td>\n<\/tr>\n
M7511<\/span><\/td>\nMitoTracker\u00ae Orange CM-H2TMRos\u00a0\u6a59\u8272<\/span><\/td>\n554\u2044576<\/span><\/td>\nYes<\/span><\/td>\nReduced<\/span><\/td>\n<\/tr>\n
M7512<\/span><\/td>\nMitoTracker\u00ae Red CMXRos\u00a0\u7ea2\u8272<\/span><\/td>\n579\/599<\/span><\/td>\nYes<\/span><\/td>\nOxidized<\/span><\/td>\n<\/tr>\n
M7513<\/span><\/td>\nMitoTracker\u00ae Red CM-H2XRos\u00a0\u7ea2\u8272<\/span><\/td>\n579\/599<\/span><\/td>\nYes<\/span><\/td>\nReduced<\/span><\/td>\n<\/tr>\n
Carbocyanine-based MitoTracker Probes<\/span><\/td>\n<\/tr>\n
M7514<\/span><\/td>\nMitoTracker\u00ae Green FM\u00a0\u7eff\u8272<\/span><\/td>\n490\/516<\/span><\/td>\nNo<\/span><\/td>\nN\/A<\/span><\/td>\n<\/tr>\n
M22425<\/span><\/td>\nMitoTracker\u00ae\u00a0Red FM\u7ea2\u8272<\/span><\/td>\n581\/644<\/span><\/td>\nNo<\/span><\/td>\nN\/A<\/span><\/td>\n<\/tr>\n
M22426<\/span><\/td>\nMitoTracker\u00ae Deep Red FM\u00a0\u6df1\u7ea2\u8272<\/span><\/td>\n644\/665<\/span><\/td>\nYes<\/span><\/td>\nN\/A<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3010\u6ce81\u3011\uff1aMitoTracker\u00ae Green FM\u00a0\uff08#M7514\uff09\u5728\u6c34\u6eb6\u6db2\u4e2d\u65e0\u8367\u5149\uff1b<\/span><\/p>\n

\u3010\u6ce82\u3011\uff1aMitoTracker\u00ae Orange CM-H2TMRos\uff08#M7511\uff09\uff0cMitoTracker\u00ae Red CM-H2XRos\uff08#M7513\uff09\u4e3a\u8fd8\u539f\u6001\u63a2\u9488\uff0c\u65e0\u8367\u5149\u3002\u53ea\u6709\u88ab\u6c27\u5316\u540e\u624d\u53d1\u8367\u5149\u3002<\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u66f4\u591a\u4ea7\u54c1\u4fe1\u606f\u8bf7\u54a8\u8be2\u5ba2\u670d\uff01<\/p>\n","protected":false},"featured_media":26239,"template":"","meta":[],"product_cat":[100],"product_tag":[184],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product\/17644"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media\/26239"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=17644"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_cat?post=17644"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_tag?post=17644"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}