{"id":15388,"date":"2019-08-08T17:47:51","date_gmt":"2019-08-08T09:47:51","guid":{"rendered":"\/\/www.562part.com\/product\/frozen-ezyeasttransformationiikit\/"},"modified":"2023-03-02T16:09:23","modified_gmt":"2023-03-02T08:09:23","slug":"frozen-ezyeasttransformationiikit","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/frozen-ezyeasttransformationiikit\/","title":{"rendered":"ZYMO Frozen-EZ YeastTransformation II Kit Frozen-EZ\u9175\u6bcd\u8f6c\u5316\u8bd5\u5242\u76d2\u2161"},"content":{"rendered":"

Frozen-EZ\u9175\u6bcd\u8f6c\u5316\u8bd5\u5242\u76d2\u2161<\/span><\/strong><\/p>\n

\"\"<\/a><\/p>\n

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\n\u7b80\u5355\uff1a\u7528\u8f7d\u4f53DNA\u5728\u4e0d\u52301\u5c0f\u65f6\u7684\u65f6\u95f4\u5185\u7528\u5355\u4e2a\u6216\u591a\u4e2a\u8d28\u7c92\u8f6c\u5316\u7684\u7b80\u4fbf\u65b9\u6cd5\u3002<\/span>
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\n\u00a0<\/span>\u00a0<\/span>
\n\u4ea7\u54c1\u63cf\u8ff0<\/span><\/strong><\/span><\/p>\n

\u4e0e\u73b0\u6709\u65b9\u6cd5\u76f8\u6bd4\uff0cFrozen-EZ\u9175\u6bcd\u8f6c\u5316II\u8bd5\u5242\u76d2\u65e8\u5728\u4f7f\u9175\u6bcd\u8f6c\u5316\u548c\u6587\u5e93\u7b5b\u9009\u66f4\u52a0\u5bb9\u6613\u548c\u9ad8\u6548\u3002\u7528Frozen-EZ Yeast Transformation II\u8bd5\u5242\u76d2\u5236\u5907\u7684\u9175\u6bcd\u7ec6\u80de\u53ef\u7acb\u5373\u7528\u4e8e\u8f6c\u5316\uff0c\u6216\u53ef\u50a8\u5b58\uff08\u5373\u2264-70\u00b0C\uff09\u4ee5\u5907\u540e\u7528\u3002\u7528\u8be5\u8bd5\u5242\u76d2\u5236\u5907\u7684\u9175\u6bcd\u53ef\u4ee5\u7528\u73af\u72b6\u548c\u7ebf\u6027DNA\u8f6c\u5316\u3002\u53e6\u5916\uff0cFrozen-EZ\u9175\u6bcd\u8f6c\u5316II\u8bd5\u5242\u76d2\u53ef\u4e0e\u5176\u4ed6\u771f\u83cc\u4e00\u8d77\u4f7f\u7528\uff0c\u5305\u62ec\u767d\u8272\u5ff5\u73e0\u83cc\uff0c\u7c9f\u9152\u88c2\u6b96\u9175\u6bcd\u548c\u7267\u7f8a\u72ac\u3002<\/span>
\n\u00a0<\/span>
\n\u00a0<\/span>\u6280\u672f\u6307\u6807<\/span><\/strong><\/span><\/p>\n

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\n\u8f6c\u5316DNA\u8f93\u5165\u00a0 \u00a0 \u00a0 \u00a0 0.2-1.0\u5fae\u514b<\/span>
\n\u8f6c\u578b\u6548\u7387\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a010 5 – 10 6 CFU \/\u5fae\u514b\u8d28\u7c92DNA\u3002<\/span><\/p>\n

 <\/p>\n

\u5e38\u89c1\u95ee\u9898<\/span><\/strong><\/span><\/p>\n

Q1\uff1a\u8be5\u5957\u4ef6\u4e2d\u6709\u4ec0\u4e48\uff1f\u5b83\u5982\u4f55\u5de5\u4f5c\uff1f<\/span><\/p>\n

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\u5728-70\u00b0C\u4ee5\u4e0b\u50a8\u5b58\u534a\u5e74\u540e\uff0c\u8f6c\u6362\u6548\u7387\u4e0d\u4f1a\u964d\u4f4e\u30026\u4e2a\u6708\u540e\uff0c\u8f6c\u6362\u6548\u7387\u9010\u6e10\u5f00\u59cb\u4e0b\u964d\u3002<\/span><\/p>\n

Q4\uff1a\u6211\u5e94\u8be5\u5982\u4f55\u89e3\u51bb\u50a8\u5b58\u7684\u51b7\u51bb\u611f\u53d7\u6001\u7ec6\u80de\uff1f<\/span><\/p>\n

\u5728\u5ba4\u6e29\u4e0b\u89e3\u51bb\u3002<\/span><\/p>\n

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\u5728\u51b7\u51bb\u7684\u7b2c\u4e00\u4e2a\u5468\u671f\u540e\uff0c\u6211\u4eec\u901a\u5e38\u4f1a\u770b\u5230\u8f6c\u5316\u5b50\u589e\u52a010-30\uff05\u3002\u60a8\u53ef\u4ee5\u91cd\u65b0\u51b7\u51bb\u548c\u878d\u5316\u611f\u53d7\u6001\u9175\u6bcd\u7ec6\u80de3-4\u6b21\uff0c\u800c\u5bf9\u8f6c\u5316\u6548\u7387\u6ca1\u6709\u660e\u663e\u5f71\u54cd\u3002\u8fdb\u4e00\u6b65\u7684\u51bb\u878d\u5faa\u73af\u4e0d\u5229\u5730\u5f71\u54cd\u8f6c\u5316\u6548\u7387\u3002<\/span><\/p>\n

\u95ee\u98986\uff1a\u5728\u8f6c\u5316\u7684\u7b2c2\u6b65\u4e2d\uff0c\u6211\u662f\u5426\u9700\u8981\u4e25\u683c\u572830\u00b0C\u4e0b\u5b75\u80b2\uff1f<\/span><\/p>\n

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Q8\uff1a\u6211\u5e94\u8be5\u5728\u9009\u62e9\u6027\u677f\u4e0a\u6492\u591a\u5c11\u8f6c\u5316\u6df7\u5408\u7269\uff1f<\/span><\/p>\n

\u5bf9\u4e8e\u5927\u591a\u6570\u73af\u5f62\u8d28\u7c92\u8f6c\u5316\uff0c50 \u00b5l\u5c31\u8db3\u591f\u4e86\u3002\u4f46\u662f\uff0c\u5982\u679c\u60a8\u4f7f\u7528\u7ebf\u6027\u5316DNA\u6216\u4f7f\u7528\u591a\u4e2a\u9009\u62e9\u6807\u8bb0\uff0c\u5219\u53ef\u4ee5\u5728\u6bcf\u5757\u677f\u4e0a\u4e0a\u6837\u591a\u8fbe200 \u00b5l\u7684\u8f6c\u5316\u6df7\u5408\u7269\uff0c\u4ee5\u589e\u52a0\u8f6c\u5316\u4f53\u7684\u6570\u91cf\u3002\u8f6c\u5316\u5b50\u7684\u6570\u91cf\u968f\u7740\u65bd\u52a0\u5230\u6bcf\u4e2a\u677f\u4e0a\u7684\u8f6c\u5316\u6df7\u5408\u7269\u7684\u6570\u91cf\u7ebf\u6027\u589e\u52a0\u3002<\/span><\/p>\n

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Q10\uff1a\u6211\u9700\u8981\u6dfb\u52a0\u8f7d\u4f53DNA\u5417\uff1f<\/span><\/p>\n

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 <\/p>\n

\u5f15\u6587<\/span><\/strong><\/p>\n

Plasmids were transformed with high efficiency using the Frozen-EZ Yeast Transformation II Kit in the construction of synthetic yeast promoters. Researchers showed that decreasing nucleosome affinity correlated with increasing promoter strength and expression levels higher than native species.<\/span><\/p>\n

Curran KA, et. al. (2014) Design of synthetic yeast promoters via tuning of nucleosome architecture. Nat Commun. 5:4002.<\/a><\/span><\/p>\n

The Frozen-EZ Yeast Transformation II Kit was used to integrate various TEF1 promoter mutants upstream of a gene in Saccharomyces cerevisiae. Efficient transformation allowed comparison of gene activity and, therefore, determination of promoter strength.<\/span><\/p>\n

Nevoigt, E., et al. (2006) Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae. Appl Environ Microb, 72(8): 5266-5273.<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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