{"id":28584,"date":"2021-03-03T11:36:11","date_gmt":"2021-03-03T03:36:11","guid":{"rendered":"\/\/www.562part.com\/?p=28584"},"modified":"2022-02-21T15:43:24","modified_gmt":"2022-02-21T07:43:24","slug":"alkaline-phosphatase-staining-kit-ii-%e7%a2%b1%e6%80%a7%e7%a3%b7%e9%85%b8%e9%85%b6%e6%9f%93%e8%89%b2%e8%af%95%e5%89%82%e7%9b%92","status":"publish","type":"post","link":"\/\/www.562part.com\/news\/28584\/","title":{"rendered":"Alkaline Phosphatase Staining Kit II \u78b1\u6027\u78f7\u9178\u9176\u67d3\u8272\u8bd5\u5242\u76d2"},"content":{"rendered":"

Alkaline Phosphatase Staining Kit II \u78b1\u6027\u78f7\u9178\u9176\u67d3\u8272\u8bd5\u5242\u76d2<\/strong><\/span><\/p>\n

\uff08Stemgent 00-0055\uff09<\/span><\/p>\n

\u3010\u6e29\u99a8\u63d0\u793aI\u3011\uff1a\u89c1\u78b1\u6027\u78f7\u9178\u9176\u4ea7\u54c1\u4e13\u9898<\/a><\/strong><\/u>\uff0c\u4e86\u89e3\u66f4\u591a\u76f8\u5173\u4ea7\u54c1\u8d44\u6599\uff1b<\/span><\/p>\n

\u3010\u6e29\u99a8\u63d0\u793aII\u3011\uff1a\u89c1\u78b1\u6027\u78f7\u9178\u9176\u6d3b\u6027\u68c0\u6d4b\u8bd5\u5242\u76d2\u4ea7\u54c1<\/a><\/u><\/strong>\uff1b<\/span><\/p>\n

\u3010\u6e29\u99a8\u63d0\u793aIII\u3011\uff1a\u89c1\u78b1\u6027\u78f7\u9178\u9176\u5e38\u7528\u751f\u5316\u5e95\u7269\u53ca\u8bd5\u5242\u76d2\u4ea7\u54c1\uff1b<\/span><\/p>\n

\u4ea7\u54c1\u5173\u952e\u8bcd\uff1a<\/strong><\/span><\/p>\n

Phosphatase, Alkaline\u78b1\u6027\u78f7\u9178\u9176\uff1bALP\/AKP\uff1b\u5bf9\u785d\u57fa\u82ef\u915a\u78f7\u9178\u76d0\uff08pNPP\uff09\uff1b\u5076\u6c2e\u5076\u8054\u6cd5\uff1b\u663e\u8272\u6cd5\uff08\u5b9a\u91cf\uff09\uff1b\u67d3\u8272\u6cd5\uff08\u5b9a\u6027\uff09\uff1b<\/span><\/p>\n

\u7814\u7a76\u610f\u4e49\uff1a<\/strong><\/span><\/p>\n

\u78b1\u6027\u78f7\u9178\u9176\uff08ALP\uff09\u6d3b\u6027\u7684\u53d8\u5316\u4e0e\u4e00\u7cfb\u5217\u751f\u7406\u548c\u75c5\u7406\u4e8b\u4ef6\u7d27\u5bc6\u5173\u8054\uff0c\u6bd4\u5982\u9aa8\u9abc\u53d1\u80b2\u3001\u9aa8\u9abc\u76f8\u5173\u75be\u75c5\u3001\u598a\u5a20\u76f8\u5173\u75be\u75c5\u548c\u708e\u75c7\u6027\u80a0\u75c5\uff08inflammatory bowel diseases\uff09\u7b49\u3002\u6b63\u56e0\u5982\u6b64\uff0cALP\u88ab\u7528\u4f5c\u8861\u91cf\u751f\u7406\u7279\u5f81\u6216\u75be\u75c5\u7684\u53c2\u7167\u6307\u6807\u3002\u53e6\u5916\uff0cALP\u6d3b\u6027\u68c0\u6d4b\u5728\u73af\u5883\u76d1\u6d4b\u3001\u98df\u54c1\u8d28\u91cf\u63a7\u5236\u7b49\u65b9\u9762\u4e5f\u5448\u73b0\u91cd\u8981\u4f5c\u7528\u3002<\/span><\/p>\n

\u25ba\u00a0\u5e72\u7ec6\u80de\u5982\u8bf1\u5bfc\u591a\u6f5c\u80fd\u5e72\u7ec6\u80deiPS\uff0cALP\u6d3b\u6027\u5f88\u9ad8\uff0c\u5219ALP\u5e38\u88ab\u7528\u4f5ciPS\u8bf1\u5bfc\u6210\u529f\u7684\u6807\u5fd7\uff1b<\/span><\/p>\n

\u25ba\u00a0\u5206\u5316\u7684\u7ed3\u80a0\u764c\u7ec6\u80de\u5185ALP\u6d3b\u6027\u663e\u8457\u63d0\u9ad8\uff0c\u5219ALP\u5e38\u88ab\u5f53\u505a\u7ed3\u80a0\u764c\u7ec6\u80de\u5206\u5316\u7a0b\u5ea6\u7684\u6307\u6807\uff08\u5b9a\u6027\/\u5b9a\u91cf\uff09\uff1b<\/span><\/p>\n

\u25ba\u00a0\u8840\u6e05\u4e2dALP\u6c34\u5e73\u63d0\u9ad8\uff0c\u88ab\u79f0\u4f5c\u9ad8\u78b1\u6027\u78f7\u9178\u9176\u8840\u75c7\uff0c\u6b64\u75c7\u72b6\u88ab\u8ba4\u4e3a\u548c\u6076\u6027\u80c6\u7ba1\u963b\u585e\u3001\u539f\u53d1\u6027\u80c6\u7ba1\u786c\u5316\u3001\u539f\u53d1\u6027\u786c\u5316\u80c6\u7ba1\u708e\u3001\u809d\u6dcb\u5df4\u7624\u548c\u809d\u8089\u7624\u7b49\u809d\u80c6\u75be\u75c5\u5bc6\u5207\u76f8\u5173\uff1b<\/span><\/p>\n

\u25ba\u00a0ALP\u662f\u6210\u9aa8\u7ec6\u80de\u7684\u526f\u4ea7\u7269\uff0c\u8840\u6e05\u4e2dALP\u6d3b\u6027\u5347\u9ad8\u548c\u9aa8\u5934\u53d1\u80b2\u5bc6\u5207\u5173\u8054\uff1b<\/span><\/p>\n

\u57fa\u672c\u4ecb\u7ecd\uff08Stemgent\uff0c00-0055\uff09\uff1a<\/strong><\/span><\/p>\n

\u5904\u4e8e\u672a\u5206\u5316\u72b6\u6001\u7684\u80da\u80ce\u5e72\u7ec6\u80de\uff08ES\uff09\u548c\u8bf1\u5bfc\u591a\u6f5c\u80fd\u5e72\u7ec6\u80de\uff08iPS\uff09\u5178\u578b\u7279\u5f81\u4e3a\u5177\u9ad8\u6c34\u5e73\u7684\u78b1\u6027\u78f7\u9178\u9176\u8868\u8fbe\uff0c\u540c\u65f6\u7ed3\u5408\u5176\u4ed6\u7684\u4e00\u4e9b\u8868\u9762\u6807\u5fd7\u7269\uff0c\u6765\u663e\u793a\u672a\u5206\u5316\u7ec6\u80de\u5177\u81ea\u6211\u66f4\u65b0\u7684\u6f5c\u80fd\u3002\u78b1\u6027\u78f7\u9178\u9176\uff08ALP\uff0c\u6216AP\uff09\u662f\u4e00\u79cd\u6c34\u89e3\u9176\uff0c\u78b1\u6027\u6761\u4ef6\u4e0b\u80fd\u591f\u5c06\u6838\u82f7\u9178\u3001\u86cb\u767d\u3001\u753e\u9187\u7b49\u5206\u5b50\u53bb\u78f7\u9178\u5316\u4f5c\u7528\u3002<\/span><\/p>\n

\u7ed3\u679c\u5448\u73b0\uff1a<\/strong><\/span><\/p>\n

\u56fa\u5b9aES\u6216iPS\u7528\u78b1\u6027\u78f7\u9178\u9176\u67d3\u8272\u8bd5\u5242\u76d2\uff08Stemgent\uff0c00-0055\uff09\u67d3\u8272\u540e\uff0c\u672a\u5206\u5316\u7ec6\u80de\u5448\u7ea2\u8272\u6216\u7d2b\u8272\uff0c\u800c\u5206\u5316\u7ec6\u80de\u65e0\u8272\u3002<\/span><\/p>\n

\u4ea7\u54c1\u7279\u6027\uff1a<\/strong><\/span><\/p>\n

1\uff09\u8bd5\u5242\u76d2\u7ec4\u5206<\/span><\/p>\n\n\n\n\n\n\n\n
\u7ec4\u5206\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u7f16\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
Fix Solution<\/span><\/td>\n25 ml<\/span><\/td>\n09-0042<\/span><\/td>\n<\/tr>\n
AP Staining Solution A<\/span><\/td>\n10 ml<\/span><\/td>\n09-0043<\/span><\/td>\n<\/tr>\n
AP Staining Solution B<\/span><\/td>\n10 ml<\/span><\/td>\n09-0065<\/span><\/td>\n<\/tr>\n
AP Staining Solution C<\/span><\/td>\n10 ml<\/span><\/td>\n09-0066<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

2\uff09\u4fdd\u5b58\u548c\u7a33\u5b9a\u6027<\/span><\/p>\n

4\u00b0C\u4fdd\u5b58\uff0c\u4e0d\u53ef\u51bb\u878d\u3002\u6309\u7167\u6307\u793a\u4fdd\u5b58\u6536\u5230\u8d27\u540e\u81f3\u5c11\u80fd\u5b58\u653e6\u4e2a\u6708\u3002<\/span><\/p>\n

3\uff09\u8d28\u91cf\u63a7\u5236<\/span><\/p>\n

\u672c\u78b1\u6027\u78f7\u9178\u9176\u67d3\u8272\u8bd5\u5242\u76d2\u4ee5\u4eba\u548c\u5c0f\u9f20\u5e72\u7ec6\u80de\u4e3a\u5bf9\u8c61\u8fdb\u884c\u529f\u80fd\u6d4b\u8bd5\uff0c\u4ee5\u786e\u4fdd\u4ea7\u54c1\u8d28\u91cf\u3002\uff08The AP Staining Kit II is functionally tested on human and mouse ES cells to ensure product quality.\uff09<\/span><\/p>\n

\u00a0\"\"<\/span><\/p>\n

\u8ba2\u8d2d\u4fe1\u606f\uff1a<\/strong>Stemgent\u539f\u88c5\u8fdb\u53e3\uff0c\u5e72\u7ec6\u80de\u7814\u7a76\u4e13\u5bb6\u7cbe\u54c1\uff0c\u54c1\u8d28\u8ba4\u8bc1\uff0c\u6b22\u8fce\u9009\u8d2d\u3002<\/span><\/p>\n\n\n\n\n
\n

\u54c1\u724c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/p>\n<\/td>\n

\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u8d27\u53f7\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0<\/span><\/td>\n\u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u76ee\u5f55\u4ef7\uff08\u5143\uff09\u00a0<\/span><\/td>\n\u8d27\u671f\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
Stemgent<\/span><\/td>\nAlkaline Phosphatase Staining Kit II<\/span><\/td>\n00-0055<\/span><\/td>\n50 assays<\/span><\/td>\n2000<\/span><\/td>\n\n

\u73b0\u8d27<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

00-0055 Datasheet<\/u><\/strong><\/span><\/p>\n

\u76f8\u5173\u4fe1\u606f\uff1aMKbio\u81ea\u4ea7<\/strong>\u78b1\u6027\u78f7\u9178\u9176\u67d3\u8272\u8bd5\u5242\u76d2\uff0c\u5728\u4fdd\u8bc1\u4f18\u79c0\u8d28\u91cf\u7684\u60c5\u51b5\u4e0b\uff0c\u63d0\u4f9b\u66f4\u6709\u7ade\u4e89\u529b\u7684\u4ef7\u683c\uff0c\u6b22\u8fce\u9009\u8d2d\u3002<\/span><\/p>\n\n\n\n\n\n
\n

\u54c1\u724c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0<\/span><\/p>\n<\/td>\n

\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0<\/span><\/td>\n\u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u76ee\u5f55\u4ef7\uff08\u5143\uff09\u00a0<\/span><\/td>\n\u8d27\u671f\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
\n

MKbio<\/span><\/p>\n<\/td>\n

\n

Alkaline Phosphatase Staining Kit<\/span><\/p>\n

\u78b1\u6027\u78f7\u9178\u9176\u67d3\u8272\u8bd5\u5242\u76d2<\/span><\/p>\n<\/td>\n

\n

MP7503-50T<\/span><\/p>\n<\/td>\n

50T<\/span><\/td>\n1900<\/span><\/td>\n\n

\u73b0\u8d27<\/span><\/p>\n<\/td>\n<\/tr>\n

MKbio<\/span><\/td>\nMP7503-100T<\/span><\/td>\n100T<\/span><\/td>\n3700<\/span><\/td>\n\n

\u73b0\u8d27<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u53c2\u8003\u6587\u732e\uff1a<\/strong><\/span><\/p>\n

Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.<\/a><\/span><\/p>\n

Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA\/LIF) and mouse development. Dev. Biol. 151: 339-351.<\/a><\/span><\/p>\n

Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.<\/a><\/span><\/p>\n

Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.<\/a><\/span><\/p>\n

Additional Publications<\/strong><\/span><\/p>\n

Momcilovic O; Sivapatham R; Oron TR; Meyer M; Mooney S; Rao MA; Zeng X. “Derivation, characteriazation, and neural differentiation of integration-free induced pluripotent stem cell lines from Parkinson’s disease patients cfarrying SNCA, LRRK2, PARK2, and GBA mutations.” PLoS ONE 11(5): e0154890. doi:10.1371\/journal.pone.0154890 (2016)<\/a><\/span><\/p>\n

\u00a0\u2014 \u2014 Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\u6240\u6709\u3011<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u78b1\u6027\u78f7\u9178\u9176\uff08ALP\uff09\u6d3b\u6027\u7684\u53d8\u5316\u4e0e\u4e00\u7cfb\u5217\u751f\u7406\u548c\u75c5\u7406\u4e8b\u4ef6\u7d27\u5bc6\u5173\u8054\uff0cALP\u88ab\u7528\u4f5c\u8861\u91cf\u751f\u7406\u7279\u5f81\u6216\u75be\u75c5\u7684\u53c2\u7167\u6307\u6807\u3002\u53e6\u5916\uff0cALP\u6d3b\u6027\u68c0\u6d4b\u5728\u73af\u5883\u76d1\u6d4b\u3001\u98df\u54c1\u8d28\u91cf\u63a7\u5236\u7b49\u65b9\u9762\u4e5f\u5448\u73b0\u91cd\u8981\u4f5c\u7528\u3002\u96c6\u5947\u751f\u7269\u4ee3\u7406\u9500\u552e\u539f\u88c5\u6b63\u54c1stemgent\u4ea7\u54c1\uff0c\u73b0\u8d27\u4f9b\u5e94\uff01<\/p>\n","protected":false},"author":1,"featured_media":28583,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,51],"tags":[],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/28584"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/comments?post=28584"}],"version-history":[{"count":3,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/28584\/revisions"}],"predecessor-version":[{"id":29545,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/28584\/revisions\/29545"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media\/28583"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=28584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/categories?post=28584"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/tags?post=28584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}