{"id":16628,"date":"2019-08-09T17:19:52","date_gmt":"2019-08-09T09:19:52","guid":{"rendered":"\/\/www.562part.com\/?p=16628"},"modified":"2023-03-13T10:43:14","modified_gmt":"2023-03-13T02:43:14","slug":"16628","status":"publish","type":"post","link":"\/\/www.562part.com\/news\/16628\/","title":{"rendered":"Dispase\u00ae II \u5206\u6563\u9176II\uff08neutral protease, grade II\uff09"},"content":{"rendered":"

\"\"<\/a><\/span><\/p>\n

\u4ea7\u54c1\u540d\u79f0 \uff1a<\/strong><\/span><\/p>\n

\u4e2d\u6027\u86cb\u767d\u9176\/\u5206\u6563\u9176II\/Neutral protease\/Dispase II<\/span><\/p>\n

\u82f1\u6587\u540c\u4e49\u540d\uff1a<\/strong><\/span><\/p>\n

Dissociating enzyme, neutral protease, tissue dissociating<\/span><\/p>\n

\u4ea7\u54c1\u63cf\u8ff0\uff1a<\/strong><\/span><\/p>\n

\u4e2d\u6027\u86cb\u767d\u9176\uff08Neutral protease\uff09\uff0c\u5546\u4e1a\u540d\u79f0Dispase II\uff0c\u4e5f\u79f0\u5206\u6563\u9176II\uff0c\u666e\u904d\u7528\u4e8e\u4e0d\u540c\u7ec4\u7ec7\u548c\u5668\u5b98\u4e2d\u7684\u7ec6\u80de\u5206\u79bb\u3002Dispase II\u662f\u4e00\u79cd\u975e\u7279\u5f02\u6027\u91d1\u5c5e\u86cb\u767d\u9176\uff0c\u80fd\u5feb\u901f\u6709\u6548\u4e14\u6e29\u548c\u7684\u7834\u574f\u7ec4\u7ec7\u7ec6\u80de\u5916\u57fa\u8d28\u91ca\u653e\u5355\u7ec6\u80de\u57f9\u517b\u7269\uff08\u539f\u4ee3\u7ec6\u80de\u57f9\u517b\u7269\uff09\u6216\u6536\u96c6\u5df2\u57f9\u517b\u7684\u7ec6\u80de\u5c06\u5176\u8f6c\u79fb\u5230\u65b0\u7684\u57f9\u517b\u57fa\u8d28\u4e0a\uff08\u4f20\u4ee3\u57f9\u517b\uff09\u3002\u53e6\u5916\uff0c\u8fd8\u80fd\u9884\u9632\u60ac\u6d6e\u7ec6\u80de\u57f9\u517b\u8fc7\u7a0b\u4e2d\u53d1\u751f\u7684\u6210\u56e2\u73b0\u8c61\u3002<\/span><\/p>\n

\u4ea7\u54c1\u7279\u70b9\uff08\u4f18\u52bf\uff09\uff1a<\/strong><\/span><\/p>\n

% \u5feb\u901f\u6709\u6548\u4f46\u6e29\u548c\u86cb\u767d\u9176\uff0c\u57fa\u672c\u5bf9\u91ca\u653e\u7ec6\u80de\u65e0\u4f24\u5bb3\uff1b<\/span><\/p>\n

% \u7ef4\u6301\u7ec6\u80de\u819c\u5b8c\u6574\u6027\uff1b<\/span><\/p>\n

% \u975e\u52a8\u7269\u6765\u6e90\uff08\u7ec6\u83cc\u6765\u6e90\uff09\uff0c\u65e0\u652f\u539f\u4f53\u548c\u52a8\u7269\u75c5\u6bd2\u6c61\u67d3\uff1b<\/span><\/p>\n

% \u6781\u5176\u7a33\u5b9a\uff0c\u4e0d\u4f1a\u53d7\u6e29\u5ea6\u3001pH\u548c\u8840\u6e05\u5185\u5bb9\u7269\u7684\u5e72\u6270\uff1b<\/span><\/p>\n

% \u901a\u8fc7\u87af\u5408\u5242\u6216\u7a00\u91ca\u5f88\u5bb9\u6613\u5931\u6d3b\uff1b<\/span><\/p>\n

\u4ea7\u54c1\u7279\u6027\uff1a<\/strong><\/span><\/p>\n

1\uff09 \u6bd4\u6d3b\u529b\uff1a\u22650.8 units\/mg protein (37 \u00b0C, casein as substrate, pH 7.5)<\/span><\/p>\n

2\uff09 \u9176\u6d3b\u529b\u5355\u4f4d\u5b9a\u4e49\uff1aOne unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 \u03bcmol (181 \u03bcg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 \u00b0C).<\/span><\/p>\n

3\uff09 \u6700\u9002pH\uff1a6.0 – 8.5<\/span><\/p>\n

4\uff09 \u6fc0\u6d3b\u5242\uff1aOptimal Ca2+concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.<\/span><\/p>\n

5\uff09 \u6291\u5236\u5242\uff1aEDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum<\/span><\/p>\n

6\uff09 \u5efa\u8bae\u5de5\u4f5c\u6d53\u5ea6\uff1a0.6 to 2.4 U\/ml<\/span><\/p>\n

7\uff09 \u4ea7\u54c1\u72b6\u6001\uff1a\u975e\u65e0\u83cc\u51bb\u5e72\u7c89<\/span><\/p>\n

\u50a8\u5b58\u6db2\u548c\u5de5\u4f5c\u6db2\u5236\u5907\u65b9\u6cd5\uff1a<\/strong><\/span><\/p>\n

1\uff09\u50a8\u5b58\u6db2\u5236\u5907\uff1a\u7528Hepe\u7f13\u51b2\u76d0\u6eb6\u6db2\uff0850 mM Hepes\/KOH pH7.4, 150 mM NaCl\uff09\u6eb6\u89e3Dispase II\u51bb\u5e72\u7c89\uff0c\u914d\u5236\u621010mg\/ml\u7684\u50a8\u5b58\u6db2\uff0c\u75280.22\u00b5M\u6ee4\u819c\u8fc7\u6ee4\u9664\u83cc\u3002\u672c\u50a8\u5b58\u6db2\u7f6e\u4e8e2-8\u2103\uff0c2\u5468\u7a33\u5b9a\uff1b\u6839\u636e\u5355\u6b21\u7528\u91cf\u5206\u88c5\u51bb\u5b58\uff0c\u9ad8\u8fbe2\u4e2a\u6708\u7a33\u5b9a\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/span><\/p>\n

2\uff09\u5de5\u4f5c\u6db2\u5236\u5907\uff1a\u4f7f\u7528\u65f6\u7528\u5408\u9002\u7684\u7ec6\u80de\u57f9\u517b\u6db2\u5c06\u50a8\u5b58\u6db2\u7a00\u91ca\u5230\u5de5\u4f5c\u6db2\u6d53\u5ea6\u5373\u53ef\uff0c\u5e38\u7528\u5de5\u4f5c\u6d53\u5ea6\u4e3a0.6-2.4 U\/ml<\/a>\u3002\u4e0d\u63a8\u8350\u4f7f\u7528>2.4 U\/ml\u7684\u5de5\u4f5c\u6d53\u5ea6\u3002\u5177\u4f53\u4f7f\u7528\u6d53\u5ea6\u8bf7\u6839\u636e\u81ea\u8eab\u5b9e\u9a8c\u4f53\u7cfb\u6216\u53c2\u8003\u6587\u732e\u6765\u8c03\u6574\u3002<\/span><\/p>\n

\u50a8\u5b58\u548c\u8fd0\u8f93\u6761\u4ef6\uff1a<\/strong><\/span><\/p>\n

\u51bb\u5e72\u7c892\uff5e8\u2103\u4fdd\u5b58\uff0c\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

Dispase\u00ae II \u5206\u6563\u9176II\uff08neutral protease, grade II\uff09<\/strong><\/span><\/p>\n

\u4ea7\u54c1\u8ba2\u8d2d\uff1a<\/strong>Roche\u539f\u88c5\u6b63\u54c1\uff0c\u5df2\u63d0\u4f9b\u7ed9\u5168\u56fd\u5404\u5730\u79d1\u7814\u5b9e\u9a8c\u5ba4\uff0c\u8d28\u91cf\u4fdd\u8bc1\uff0c\u6b22\u8fce\u54a8\u8be2\u3002<\/span><\/p>\n\n\n\n\n\n\n\n
\n

\u54c1\u540d <\/strong><\/span><\/p>\n<\/td>\n

\n

\u00a0 \u4ea7\u5730 <\/strong><\/span><\/p>\n<\/td>\n

\n

\u8d27\u53f7 <\/strong><\/span><\/p>\n<\/td>\n

\n

\u89c4\u683c <\/strong><\/span><\/p>\n<\/td>\n

\n

\u5355\u4ef7\uff08\u5143\uff09 <\/strong><\/span><\/p>\n<\/td>\n

\n

\u5907\u6ce8 <\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Dispase\u00ae II (neutral protease, grade II)<\/a><\/span><\/p>\n<\/td>\n

\n

\u00a0 Roche<\/span><\/p>\n<\/td>\n

\n

04942078001<\/span><\/a><\/p>\n<\/td>\n

\n

1 00MG<\/span><\/p>\n<\/td>\n

\n

\u00a0 \u00a0 \u00a0580<\/span><\/p>\n<\/td>\n

\n

\u73b0\u8d27<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Dispase\u00ae II (neutral protease, grade II)<\/a><\/span><\/p>\n<\/td>\n

\n

\u00a0 Roche<\/span><\/p>\n<\/td>\n

\n

04942078001<\/span><\/a><\/p>\n<\/td>\n

\n

\u00a01G<\/span><\/p>\n<\/td>\n

\n

\u00a0 \u00a01980<\/span><\/p>\n<\/td>\n

\n

\u73b0\u8d27<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Dispase II \u5206\u6563\u9176II\uff08\u4e2d\u6027\u86cb\u767d\u9176\uff09<\/a><\/span><\/p>\n<\/td>\n

\n

\u00a0 MKBIO<\/span><\/p>\n<\/td>\n

\n

MX1006-100MG<\/span><\/p>\n<\/td>\n

\n

\u00a0100MG<\/span><\/p>\n<\/td>\n

\n

\u00a0 \u00a0 \u00a0480<\/span><\/p>\n<\/td>\n

\n

\u73b0\u8d27<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Dispase II \u5206\u6563\u9176II\uff08\u4e2d\u6027\u86cb\u767d\u9176\uff09<\/span><\/p>\n<\/td>\n

\n

\u00a0 MKBIO<\/span><\/p>\n<\/td>\n

\n

MX1006-1000MG<\/span><\/p>\n<\/td>\n

\n

\u00a01G<\/span><\/p>\n<\/td>\n

\n

\u00a0 \u00a0 1700<\/span><\/p>\n<\/td>\n

\n

\u73b0\u8d27<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u63a8\u8350\u4f7f\u7528\u61cb\u5eb7\u751f\u7269\u751f\u4ea7\u63d0\u4f9b\u7684\u5206\u6563\u9176\u4ea7\u54c1\uff0c\u8d28\u91cf\u7a33\u5b9a\uff0c\u6027\u4ef7\u6bd4\u66f4\u9ad8\uff01<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u4ea7\u54c1\u540d\u79f0 \uff1a \u4e2d\u6027\u86cb\u767d\u9176\/\u5206\u6563\u9176II\/Neutral protease\/Dispase II \u82f1\u6587\u540c\u4e49\u540d\uff1a Dissociating enzyme, neutral protease, tissue dissociating \u4ea7\u54c1\u63cf\u8ff0\uff1a \u4e2d\u6027\u86cb\u767d\u9176\uff08Neu<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,51],"tags":[],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/16628"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/comments?post=16628"}],"version-history":[{"count":4,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/16628\/revisions"}],"predecessor-version":[{"id":30596,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/16628\/revisions\/30596"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=16628"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/categories?post=16628"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/tags?post=16628"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}